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KMID : 0613820030130060924
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Journal of Life Science
2003 Volume.13 No. 6 p.924 ~ p.932
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Epigallocatechin Gallate Activates Phospholipase D in Glioma Cells
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Lee Sung-Hoon
Kim Si-Yeon
Kim Joon-Mo
Min Do-Sik
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Abstract
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Epigallocatechin-3 Gallate (EGCG), a major constituent of green tea, has attracted increasing interest because of its many reported health benefits. Here we demonstrate for the first time that EGCG stimulates phospholipase D (PLD) activity in U87 human astroglioma cells. EGCG-induced PLD activation was abolished by the phospholipase C (PLC) inhibitor and a lipase inactive PLC-γ1 mutant, and was dependent on intracellular Ca²+, and possibly involved Ca²+/calmodulin-dependent protein kinase II (CaM kinase II). Interestingly, EGCG induced translocation of PLC-γ1 from the cytosol to the membrane and PLC-γ1 interaction with PLD1. Taken together, these results demonstrate for the first time that in human astroglioma cells, EGCG regulates PLD activity via a signaling pathway involving a PLC-γ1 (inositol 1,4,5-trisphosphate-Ca²+)-CaM kinase II-PLD pathway.
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KEYWORD
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EGCG, PLD, PLC-γ1, CaM kinase II
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